In the field of single cell sequencing, the microfluidic droplet technology is employed to isolate and sort single cells and to serve as reaction vessels for single cell NGS library construction. With microfluidic droplet devices, floating cells can be separated in aqueous droplets which are stabilized by surfactants in the oil phase. Subsequently, droplets containing single cells are sorted and NGS libraries are constructed in droplets.
Droplet microfluidic devices screen microbial single cells with unpredendant flexibility, ultrahigh throughput and precision.
Microfluidic droplet-based microbe screening has the potential to revolutionize traditional methods that are labor intensive, time consuming and inefficient. It can sort microbes with different phenotypes accurately and efficiently. With properly designed microfluidic channels and driving mechanisms, every microbe-containing droplet can be controlled separated and precisely, making microbe sorting and subsequent single cell sequencing possible.
Preimplantation genetic diagnosis (PGD) is the analysis of genetic materials of embryos before implantation.
PDG can be applied to detecting single gene genetic diseases, sex chromosome-linked genetic diseases, chromosomal abnormalities, mitochondrial diseases and HLA typing. Due to the lack of embryonic materials, subsequent NGS analysis is critically dependent on the whole genome amplification of single cells. Thus, microfluidic droplet-based whole genome amplification is of great value to PGD.
Microfluidic droplet manipulation can be utilized to control liquid flow with superior characteristics, such as reduced sample consumption, accelerated reaction rates and easier integration.
Due to its simplicity, convenience and enhanced enrichment, microfluidic droplet screening has increasingly been applied to circulating tumor cell (CTC) studies. The application of microfluidic chip to CTC isolation and analysis increased cell capture rates, thus facilitating genome and transcriptome study of CTCs