High Accuracy and Precision in DNA Absolute Quantification and CNV Detection
Digital PCR is the third generation of PCR technologies. It provides absolute and reproducible quantification of nucleic acid present in a sample, without the need of a calibration curve. The principle of dPCR is that PCR mix is partitioned into a large number of individual reaction partitions prior to the amplification step, resulting in either 1 or 0 targets in each unit. Following amplification, the number of positive vs negative reactions is determined and the absolute quantification of target calculated using Poisson statistics.
In comparison to traditional quantitative PCR (qPCR),
the absolute quantification by dPCR provides superior performance :
Dawei OsciDrop™ digital PCR is built upon its revolutionary and patented DAPCO™(Droplet Array Production by Cross-Interface Oscillation) technology